trypsin edta  (ATCC)

Journal: bioRxiv

Article Title: Substantial genomic and methylation variability between MCF-7 sublines

doi: 10.64898/2026.02.17.706500

Figure Lengend Snippet: Violin plots of cis-regulatory elements CpG methylation (A) and aggregate whole genome CpG methylation of both MCF-7 sublines in 10kbp intervals (B) .The y-axis represents the methylation percentage and shows the bulk of CpG sites in the ECACC subline are hypermethylated compared to the ATCC subline which has CpG sites with a wide range of methylation levels. Similarly, all regulatory elements in the ECACC sublines are hypermethylated compared to the ATCC subline.

Article Snippet: We note that as both samples have relatively similar coverage (x28 ATCC vs x30 ECACC), these differences are unlikely to arise from differences in sequencing coverage, which may influence methylation call accuracy ( ).

Techniques: CpG Methylation Assay, Methylation

Journal: bioRxiv

Article Title: Substantial genomic and methylation variability between MCF-7 sublines

doi: 10.64898/2026.02.17.706500

Figure Lengend Snippet: Ridge plots for CpG methylation in retrotransposons, including long interspersed element 1 LINE-1 families, Alu and SVA, compared between sublines. The ridges represent the distribution of methylation for each subfamily. The range on the x-axis corresponds to the level of methylation, where 0 represents no methylation and 1 represents complete methylation. Full-length L1 elements (A) and their CpG-rich 5’ UTRs (B) across multiple subfamilies show substantial differential methylation between ATCC and ECACC derived cell lines. This differential methylation is also present in Alu (C) and SVA elements (D) from various subfamilies to a lesser extent.

Article Snippet: We note that as both samples have relatively similar coverage (x28 ATCC vs x30 ECACC), these differences are unlikely to arise from differences in sequencing coverage, which may influence methylation call accuracy ( ).

Techniques: CpG Methylation Assay, Methylation, Derivative Assay

Journal: Nanomaterials

Article Title: Identifying Key Factors Affecting mRNA-Lipid Nanoparticles Drug Product Formulation Stability

doi: 10.3390/nano16040268

Figure Lengend Snippet: Storage buffer screening against freeze–thaw for F1–F5 Fluc mRNA–LNP formulations, including ( a , b ) particle size, ( c , d ) polydispersity index, ( e , f ) encapsulation efficiency, and ( g , h ) in vitro transfection efficiency in HEK-293 cells. All data are mean ± SD ( n ≥ 3). F—Formulation; FLuc—Firefly luciferase; mRNA–LNP—Messenger ribonucleic acid–lipid nanoparticle; PBS—Phosphate-Buffered Saline; n—Number of samples; SD—Standard deviation.

Article Snippet: HEK-293 cells (CRL-1573) procured from ATCC (Manassas, VA, USA) were grown in Dulbecco’s Modified Eagle’s Medium (DMEM, CAS# 2025-01-30).

Techniques: Encapsulation, In Vitro, Transfection, Formulation, Luciferase, Saline, Standard Deviation

Journal: Nanomaterials

Article Title: Identifying Key Factors Affecting mRNA-Lipid Nanoparticles Drug Product Formulation Stability

doi: 10.3390/nano16040268

Figure Lengend Snippet: mRNA purity % and in vitro transfection efficiency of FLuc mRNA–LNPs (F1–F4) in 20 mM and 30 mM Tris + 10% sucrose. Samples were measured at 0, 0.5, 1, 3, 6, and 12 months under four storage temperatures (−80 °C, −20 °C, 5 °C, 25 °C). mRNA purity % was measured by MCE. Transfection efficiency was performed on HEK-293 cells, and the luciferase intensity was measured at 24 h post-treatment. For transfection, the data are mean ± SD ( n ≥ 3). At 12-month timepoints, no statistical significance was observed between 20 mM and 30 mM Tris corresponding samples in transfection efficiency results. (One-way ANOVA, p -value > 0.05). For mRNA purity, the data are the means of 3 repeated measures. Note: the time-zero FLuc mRNA purity is <100% due to inherent in vitro transcribed mRNA drug substance heterogeneity and drug substance handling (one freeze–thaw cycle) prior to LNP preparation. F—Formulation; FLuc—Firefly luciferase; LNP—Lipid nanoparticles; MCE—Microchip capillary electrophoresis; MLI—Mean luminescence intensity; mRNA—Messenger ribonucleic acid; SD—Standard deviation.

Article Snippet: HEK-293 cells (CRL-1573) procured from ATCC (Manassas, VA, USA) were grown in Dulbecco’s Modified Eagle’s Medium (DMEM, CAS# 2025-01-30).

Techniques: In Vitro, Transfection, Luciferase, Formulation, MicroChIP Assay, Electrophoresis, Standard Deviation